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NL5 Point Scanning Confocal Microscope VIS / Microscopes imaging

The NL5 uses vintage technology from the eighties, now combined with modern cutting-edge digital technology, to create a fast confocal microscope with high resolution and perfect sensitivity. A slit pinhole, together with an extremely sensitive sCMOS camera as detector, make NL5 the perfect tool for fast and deep 3D live cell imaging

NL5 Point Scanning Confocal Microscope VIS summary

Point-scanning confocal systems are commonly used, but their speed is limited. NL5 contains a slit pinhole that increases acquisition speed dramatically by scanning a full line at a time instead of a point. Due to the speed of the scanner, your samples will not be exposed to the laser for a long time, preventing photobleaching and phototoxicity. In addition, the high quantum efficiency of the modern sCMOS camera conjoined with NL5, allows for utilizing low laser power. Therefore, a high signal-to-noise ratio image can be captured without signs of phototoxicity or bleaching.

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    Features
    • Fast scanning confocal microscope
    • High resolution and low phototoxicity
    • Ideal for deep 3D live cell imaging
    • Compact and very easy to use
    • Keep widefield capabilities with motorized bypass
    • Convert your existing widefield fluorescence microscope into a fast and highly sensitive confocal 3D imaging microscope with NL5
    Applications
    • High-content screening
    • Single cell imaging
    • Neural communication and axonal
    • transport
    • Vesicle transport
    • Protein dynamics
    • Fast live cell dynamics
    Specifications
    NL5
    DetectorCamera
    Resolution170nm (after deconvolution, raw image = 170nm)
    SensitivityUp to 95% QE
    FOV330×330µm (40x, magnification)
    Optimized for40-100x
    ScannerDigital (closed-loop)
    Speed25 fps full frame
    WavelengthVIS
    SoftwareMicromanager, Volocity, NIS Elements
    IntegrationAPI
    PSF for deconvolution withMicrovolution
    Bypass modeYes (motorized)
    slit pinhole advantages

    Image: NL-5 : slit pinhole advantages versus spinning-disk systems

    Volvox autofluorescence 561 excitation channel. Image acquired 90 microns away from the coverslip 100x 1.5 objective was used with both systems

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