Lasers & Optical Fibers Technologies
All-in-one two-photon microscope (FEMTO3D ATLAS) / Microscopy
The all-in-one solution for extremely high-speed 3D imaging
All-in-one two-photon microscope (FEMTO3D ATLAS) summary
The FEMTO3D Atlas microscope combines high-tech science, engineering and refinement in 3D measurements and technology into an all-in-one solution. The unique properties of Atlas enables its users to scan neuronal, dendritic, or other biological processes in 3D, up to a million times faster than classical scanning methods, at a preserved two photon resolution.
All your needs in the field of multiphoton imaging can be satisfied with one single system:
- 3D random-access scanning
- High-speed arbitrary frame scanning
- Upgrade or individual system
- 3D anti-motion technology
- 3D photostimulation with imaging
The Femto3D Atlas can be combined with a preexisting upright microscope through its adjustable, modular frame. Therefore, it can extend existing microscopes with a fast 3D functional imaging capability. To learn more about FEMTO3D Atlas microscope, upload our brochure
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- 3D random-access point
- High-speed arbitrary frame
- 3D trajectory and multiple-line
- 3D ribbon scanning
- 3D snake
- 3D multi-layer
- 3D chessboard
- 3D multi-cube
- In vitro and in vivo measurements
- Neural networks
- 3D dendritic scanning
- Calcium imaging during behavior
- Voltage imaging
- Optogenetics
- Uncaging
| FEMTO3D Atlas | ||
|---|---|---|
| Scanning technique | 3D RANDOM ACCESS POINT | HIGH-SPEED ARBITRARY FRAME |
| Speed | 30 kHz per point | 40 frame per second |
| Number of simultaneoulsy scanning regions | 2000 cells < | Oblique directioned neurons and dendrites |
| Benefits in neuroscience | 3D Networks imaging in large cell populations | Volume imaging, voltage imaging |
| Scanning technique | 3D TRAJECTORY AND MULTIPLE-LINE | 3D RIBBON SCANNING |
| Speed | Up to 30 kHz per spine | Up to 3 kHz on a 50 µm long dendritic segment |
| Number of simultaneoulsy scanning regions | Up to 1000 spines | Up to an 1000 µm long dendritic segment |
| Benefits in neuroscience | Dendritic imaging without interruption and recording activity of over 150 spines | Imaging of activity in over 12 spiny dendritic segments |
| Scanning technique | 3D SNAKE | 3D MULTI-LAYER |
| Speed | Up to 300 Hz on a 50 um long dendritic segment | Up to 3 kHz per 2D ROI |
| Number of simultaneoulsy scanning regions | Up to an 300 um long dendritic segment | Up to 300 regions |
| Benefits in neuroscience | Dendritic imaging during large amplitude movements | Imaging along the entire length of the cell |
| Scanning technique | 3D CHESSBOARD | 3D MULTI-CUBE |
| Speed | Up to 3 kHz per 2D ROI | Up to 300 Hz per volume |
| Number of simultaneoulsy scanning regions | Up to 300 regions | Up to 300 volumes |
| Benefits in neuroscience | High speed somatic recordings, network imaging | Imaging of somata during large amplitude movements |
FEMTO3D Atlas uses electrically tunable acousto-optic deflectors (AODs) as scanners, directing the laser beam in X, Y and Z directions. It makes the positioning speed of the focal spot equivalent in all directions, and independent of the distance.
Those deflectors do not contain mechanical components (e.g. scanning mirrors), which would slow the movement or disturb the model animal.
