Slit scanning confocal systems are commonly used, but their speed is limited. NL5 contains a slit pinhole that increases acquisition speed dramatically by scanning a full line at a time instead of a point. Due to the speed of the scanner, your samples will not be exposed to the laser for a long time, preventing photobleaching and phototoxicity. In addition, the high quantum efficiency of the modern sCMOS camera conjoined with NL5, allows for utilizing low laser power. Therefore, a high signal-to-noise ratio image can be captured without signs of phototoxicity or bleaching.
The new NL5+ module have been upgraded with a motorized 6 positions filters which improve its speed from 25 fps to 75 fps!
for more informations
|Emission filters||Not installed (upgradable)||Motorized 6 positions|
|Speed||25 fps (full frame)||75 fps (2048x512) & 55 fps (2048x2048)|
|with camera Kinetix Teledyne|
|Detector||Camera (sCMOS)||Camera (sCMOS)|
|Resolution||170nm (after deconvolution)||170nm (after deconvolution)|
|Sensitivity||Up to 95% QE||Up to 95% QE|
|FOV||330×330µm (40x, magnification)||330×330µm (40x, magnification)|
|Scanner||Digital (closed-loop)||Digital (closed-loop)|
|Wavelength||VIS (400-700)||VIS (400-700)|
|Software||Micromanager, Inscoper, Volocity, NIS Elements||Micromanager, Inscoper, Volocity, NIS Elements|
|PSF for deconvolution with||Microvolution||Microvolution|
|Bypass mode||Yes (motorized)||Yes (motorized)|
Slit pinhole advantages versus spinning-disk systems:
Volvox autofluorescence 561 excitation channel. Image acquired 90 microns away from the coverslip 100x 1.5 objective was used with both systems.