Slit scanning confocal systems are commonly used, but their speed is limited. NL5 contains a slit pinhole that increases acquisition speed dramatically by scanning a full line at a time instead of a point. Due to the speed of the scanner, your samples will not be exposed to the laser for a long time, preventing photobleaching and phototoxicity. In addition, the high quantum efficiency of the modern sCMOS camera conjoined with NL5, allows for utilizing low laser power. Therefore, a high signal-to-noise ratio image can be captured without signs of phototoxicity or bleaching.
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NL5 | |
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Detector | Camera |
Resolution | 170nm (after deconvolution, raw image = 170nm) |
Sensitivity | Up to 95% QE |
FOV | 330×330µm (40x, magnification) |
Optimized for | 40-100x |
Scanner | Digital (closed-loop) |
Speed | 25 fps full frame |
Wavelength | VIS |
Software | Micromanager, Volocity, NIS Elements |
Integration | API |
PSF for deconvolution with | Microvolution |
Bypass mode | Yes (motorized) |
Slit pinhole advantages versus spinning-disk systems:
Volvox autofluorescence 561 excitation channel. Image acquired 90 microns away from the coverslip 100x 1.5 objective was used with both systems.