RCM1 is based on the Re-scan Confocal Microscope (RCM) principle. Capture datasets with 170nm raw resolution (120nm after deconvolution) by using 60x to 100x high numerical aperture (NA) magnification objectives and keep the laser intensity at a minimum. RCM1 acquires super-resolution images with only 85 nano-watts laser power.
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|Resolution||120nm (after deconvolution, raw image = 170nm)|
|Sensitivity||Up to 95% QE|
|FOV||130×130µm (60x, super-resolution)|
|Optimized for||100x, 60x (high NA)|
|Speed||1fps @ 512x512 pixels|
|Wavelength||VIS or NIR|
|Software||Micromanager, NIS Elements|
|PSF for deconvolution with||Microvolution, SVI Huygens|
Hela cell stained for DNA (blue), Actin (green), Tubulin (red) and clathrin (yellow). Captured on RCM1 using a 100x 1.45 objective. Sample courtesy: Nicolas Touret, University of Alberta, Canada.