RCM2 has optics to make it suitable for super-resolution imaging with high NA objectives in the low magnification range, like 40x 1.4. A lower magnification allows for a bigger field of view (FOV), brighter images, and even lower laser power. RCM2 has demonstrated imaging at 10 nano-watt excitation power!
Improvements of sCMOS cameras allow you to sample resolution of low magnification objectives effectively, without increasing the exposure time. In a regular PMT-based confocal this is not possible.
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|Resolution||120nm (after deconvolution, raw image = 170nm)|
|Sensitivity||Up to 95% QE|
|FOV||220×220µm (40x, super-resolution)|
|Optimized for||100x, 60x, 40x (high NA)|
|Speed||2fps @ 512×512 pixels|
|Software||Micromanager, Volocity, NIS Elements, Zen, LAS X, Cellsens|
|Integration||Hardware – USB connection|
|PSF for deconvolution with||Microvolution, SVI Huygens|
Neurons in co-culture stained for Actin (red), MAP2 (magenta) and Tau (green). Imaged on RCM2 with a 40x 1.4 objective. Sample courtesy: Vera Wiersma, VU University, Amsterdam, the Netherlands.